Simply add the specially formulated Agarose Dissolving Buffer (ADB) to the gel slice containinga DNA sample, let dissolve, and then transfer to the supplied ZymoSpin™ IC-XL Column. There is no need for organic denaturants or chloroform. Instead, the product utilizes unique spin column technology to yield high-quality, purified DNA in just minutes. DNA purified using the Zymoclean™ Large Fragment DNA Recovery Kit is ideal for PCR, sequencing, endonuclease digestion, ligation, etc. The entire procedure typically takes about 15 minutes.